{"id":483,"date":"2013-09-18T00:00:00","date_gmt":"2013-09-17T21:00:00","guid":{"rendered":"https:\/\/uomosul.edu.iq\/science\/2013\/09\/18\/%d8%ac%d8%b1%d8%aa-%d9%85%d9%86%d8%a7%d9%82%d8%b4%d8%a9-%d8%a7%d8%b7%d8%b1%d9%88%d8%ad%d8%a9-%d8%a7%d9%84%d8%af%d9%83%d8%aa%d9%88%d8%b1%d8%a7%d9%87-%d9%84%d9%84%d8%b7%d8%a7%d9%84%d8%a8%d8%a9-%d8%b2\/"},"modified":"2013-09-18T00:00:00","modified_gmt":"2013-09-17T21:00:00","slug":"%d8%ac%d8%b1%d8%aa-%d9%85%d9%86%d8%a7%d9%82%d8%b4%d8%a9-%d8%a7%d8%b7%d8%b1%d9%88%d8%ad%d8%a9-%d8%a7%d9%84%d8%af%d9%83%d8%aa%d9%88%d8%b1%d8%a7%d9%87-%d9%84%d9%84%d8%b7%d8%a7%d9%84%d8%a8%d8%a9-%d8%b2","status":"publish","type":"post","link":"https:\/\/uomosul.edu.iq\/science\/%d8%ac%d8%b1%d8%aa-%d9%85%d9%86%d8%a7%d9%82%d8%b4%d8%a9-%d8%a7%d8%b7%d8%b1%d9%88%d8%ad%d8%a9-%d8%a7%d9%84%d8%af%d9%83%d8%aa%d9%88%d8%b1%d8%a7%d9%87-%d9%84%d9%84%d8%b7%d8%a7%d9%84%d8%a8%d8%a9-%d8%b2\/","title":{"rendered":"\u062c\u0631\u062a \u0645\u0646\u0627\u0642\u0634\u0629 \u0627\u0637\u0631\u0648\u062d\u0629 \u0627\u0644\u062f\u0643\u062a\u0648\u0631\u0627\u0647 \u0644\u0644\u0637\u0627\u0644\u0628\u0629 \u0632\u0647\u0631\u0627\u0621 \u0645\u062d\u0645\u062f \u0639\u0644\u064a \u0627\u062d\u0645\u062f \u062d\u0645\u0648\u062f\u0627\u062a \u0641\u064a \u0642\u0633\u0645 \u0627\u0644\u0643\u064a\u0645\u064a\u0627\u0621 \/ \u0643\u0644\u064a\u0629 \u0627\u0644\u0639\u0644\u0648\u0645 \/ \u0648\u062d\u0635\u0644\u062a \u0639\u0644\u0649 \u0627\u0644\u0639\u062f\u064a\u062f \u0645\u0646 \u0627\u0644\u0627\u0633\u062a\u0646\u062a\u0627\u062c\u0627\u062a"},"content":{"rendered":"<ul>\n<li>The number of rheumatoid arthritis females was more than males and most common after age of 40thand 50thyears.\n<li>The number of patients which living in urban areas was higher than those in rural areas.\n<li>Physical and chemical analysis of SF revealed more than 1 ml, yellow green color, cloudy, low viscosity, with large fat droplets, bloody fluid, low pH degree, low glucose level, high total protein and albumin levels\n<li>The reference value of latent-MMP-1 activity level was in the range of (47.38- 227.72) ng\/ml.\n<li>Also, the activity level of enzyme was significantly different between the synovial fluid and serum of patients with RA and between the serum of patient&rsquo;s RA and the serum of control.\n<li>Factors of age, gender and geographic place had no impact on the activity level of Latent MMP-1, while smoking factor had an impact on the activity level of enzyme in RA patients and control.\n<li>There were highly significant levels of sialic acid, MDA and significantly low levels of hyaluronidase activity, GSH, vitamin C in synovial fluid when compared with the serum of RA patients. Moreover, there was a highly significant increase levels of hyaluronidas, ALP, lactate dehydrogenase, sialic acid, MDA, sulfate, phosphorus, in contrast to a significant decrease of GSH, vitamin C, vitamin D3, calcium and calcium\/phosphorus ratio in the serum of RA patients when compared with the serum of control.\n<li>A significant difference was found in levels activity of latent-MMP-1, CRP, hyaluronidase, ALP, sialic acid, GSH, MDA, Vitamin C, Vitamin D3, calcium, phosphorus and calcium\/phosphorus ratio concentration between severe disease activity and moderate disease activity in RA.\n<li>A positive significant correlation between latent-MMP-1 and DAS-28 in serum and synovial fluid, while a negative significant correlation of latent-MMP-1 with vitamin C and Vitamin D3 in serum of RA patients was obtained.\n<li>Two porteinous components had been isolated by gel filtration chromatography.\n<li>It was found that only the first peak has high level activity of latent MMP-1.\n<li>The molecular weight of MMP-1 serum and synovial fluid using gel filtration chromatography was found to be (47.6 and 48.2) kilo dalton respectively, and by using SDS-electrophoresis was found to be (49.6 and 50) kilo dalton in serum and synovial fluid respectively.\n<li>The appearance of the main top of the enzyme from serum and SF at retention times (1.65) minutes compared with retention time of standard latent MMP-1 at (1.571) minutes.\n<li>Finally, the results revealed that the addition of ethylene diamine tetra acetic acid, 2,4-dinitrophenol, sodium azide, potassium oxalate, mercaptoethanol, glutathione, cystein and blood serum decreased the activity level of MMP-1,\n<li>while the addition of NaCl, MgSO4, CoCl2, CaCl2, CuSO4, ZnSO4, HgCl2and Pepsin increased the activity level of latent MMP-1.<br \/>\n","protected":false},"excerpt":{"rendered":"<p>The number of rheumatoid arthritis females was more than males and most common after age of 40thand 50thyears. The number of patients which living in urban areas was higher than those in rural areas. Physical and chemical analysis of SF revealed more than 1 ml, yellow green color, cloudy, low viscosity, with large fat droplets, bloody fluid, low pH degree, low glucose level, high total protein and albumin levels The reference value of latent-MMP-1 activity level was in the range of (47.38- 227.72) ng\/ml. Also, the activity level of enzyme was significantly different between the synovial fluid and serum of patients <a href=\"https:\/\/uomosul.edu.iq\/science\/%d8%ac%d8%b1%d8%aa-%d9%85%d9%86%d8%a7%d9%82%d8%b4%d8%a9-%d8%a7%d8%b7%d8%b1%d9%88%d8%ad%d8%a9-%d8%a7%d9%84%d8%af%d9%83%d8%aa%d9%88%d8%b1%d8%a7%d9%87-%d9%84%d9%84%d8%b7%d8%a7%d9%84%d8%a8%d8%a9-%d8%b2\/\"> [\u0625\u0642\u0631\u0623 \u0627\u0644\u0645\u0632\u064a\u062f]<\/a><\/p>\n","protected":false},"author":1,"featured_media":484,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-483","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-2"],"_links":{"self":[{"href":"https:\/\/uomosul.edu.iq\/science\/wp-json\/wp\/v2\/posts\/483","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/uomosul.edu.iq\/science\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/uomosul.edu.iq\/science\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/uomosul.edu.iq\/science\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/uomosul.edu.iq\/science\/wp-json\/wp\/v2\/comments?post=483"}],"version-history":[{"count":0,"href":"https:\/\/uomosul.edu.iq\/science\/wp-json\/wp\/v2\/posts\/483\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/uomosul.edu.iq\/science\/wp-json\/wp\/v2\/media\/484"}],"wp:attachment":[{"href":"https:\/\/uomosul.edu.iq\/science\/wp-json\/wp\/v2\/media?parent=483"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/uomosul.edu.iq\/science\/wp-json\/wp\/v2\/categories?post=483"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/uomosul.edu.iq\/science\/wp-json\/wp\/v2\/tags?post=483"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}