College of Education for Pure Science

The M.Sc. Thesis Viva Launched in College of Education for Pure Science – Department of Biology entitled ” Measurement of some immune cytokines in Albino mice injected with the Protoscoleces of Echinococcus granulosus exposed to microwaves ” on Thursday, 28-4-2022,The College of Education for Pure Sciences at the University of Mosul has done the M.Sc. Thesis Viva of the student Seham Khalil Ibrahim and under the supervision of ProF Dr. Asmaa Abdulaziz Ali.The current study investigated the effect of microwaves on the viability of Protoscoleces of Echinococcus granulosus in vitro by applying different exposures 3.5.7.10.12.13.15 second at a constant frequency 50 Hz 900 W and in vivo, on the immune response of BALB/c mice against infection with cystic echinococcosis in contrast to the control group, injected with un exposure Protoscoleces, depending on many criteria including changes in the numbers, weights and diameters of hydatid cysts, changes in the weights of liver and spleen and their organ index, measurement of immune cytokines in the serum of the experimental animals.The results revealed: 1.An increase in the mortality rate of Protoscoleces in vitro,in proportional with exposure duration of microwaves.2. A reduction of the hydatid cysts numbers with ratios of 97%, 87.2% and 92.5%, in treated mice that with the viability of 30%, four, three and two months post infection, respectively.3. A Reduction significant (P≤ 0.001) in the hydatid cysts numbers, weights and diameters of treated mice, the minimum number was 0.6 in comparrson with the control group 42.2, where as the minimum weight was 0.0004 mg compared to the control 0.132 mg, the lowest diameters was 0.140 mm, where as in the control was 2.820 mm, four months post infection.4. A significant reduction (P≤ 0.001) in the liver and spleen weights, in treated mice, the minimum weight of the liver was the 1.2380 gm compared to the control 1.9800 gm with the organ index of 16.480 compared to the control 56.275, four months post infection, concerning the weights of the spleen and organ index there were no significant differences between treated and control groups, with the minimum organ index in the treated group 1.687 in comparison to the control 4.776, four months post infection.5. A significant increase (P≤ 0.001) in the immune response of treated mice, represented by a significant decrease in the levels of IL -4 8.610 pg/ml and IL -10 42.75 pg/ml in comparison with the control group 63.862 pg/ml and 251.740 pg/ml, respectively, an Increase in IFN- γ level in treat mice 140.47 pg/ml compared to the control group 51.01 pg/ml, four months post infection.The Panel was chaired by Prof Dr. Abrahim Ahmed Abdullah/ Alnoor university College, and the membership of both Prof Dr. Adel Talib Said / university of Dhouk / College of medicine and under the supervision and membership of Prof. Dr. Asmaa Abdulaziz Ali.