College of Education for Pure Science

PH.D. Dissertation Viva in the College of Education for Pure Science entitled ” Morphometrical and chemical  study of white Rat pancreas Rattus norvegicus from birth to puberty ”

The College of Education for Pure Science, University of Mosul, has done the PH.D. Dissertation Viva entitled ” Morphometrical and chemical  study of white Rat pancreas Rattus norvegicus from birth to puberty ”

On Thursday, September 7, the respected Dean of the College, Assistant Professor Dr. Qais Ismail Ibrahim, the Honorable Scientific Associate and Administrative Associate, the Honorable Head of the Department of Biology, and a number of the college’s teachers were attended the viv

This study, presented by the PH.D. Student Asmaa Ibraheem Ahmed Al-Abbaddie in the Department of Biology, was conducted to investigate the developmental morphological, histochemical and immunohistochemical changes that occurred in the pancreas of albino rats at different postnatal  ages. To achieve this goal, samples of the pancreas were used, which were collected from 72 rats of different ages. Several histological stains were used, in addition to the immunohistochemistry stain for the detection of Ki67 proliferating cells and ELISA test for insulin and glucagon level in serum

A macroscopic study of the pancreas in white rats showed clear shape of the pancreas was observed in the third group of rats (14 days old) where it was observed in the form of a white to pink gland suspended in the mesentery extending between the stomach and the duodenum and the ascending colon, and its three lobes. Whereas the histological and functional structures completed at postnatal day 21.

The islets of Langerhans in rats of the newly born kits were distinguished by their clear association with the appearance and development of the ductal system in the pancreas, where it was observed that the origin of most of the secretory ducts arises from the same cells from which the islets of Langerhans arise

Rats of 7-day-old showed a clear development in the composition of the islets of Langerhans, as these islets appeared larger in size and more numerous than in the previous group as the presence of large cells with a basal cytoplasm (blue) and a large oval nucleus was observed and their presence is concentrated in the peripheral part of the islets, which are alpha cells, while it was observed that there are other, smaller cells with cytoplasm tending to acidic (red) and small nuclei of dark color, which are found in the central part of the islets, and they were beta cells. It is noticeable at this age that the percentage of alpha cells was predominant in most of the islets of Langerhans. The results of rats at the age of 14 days showed that the number of beta cells were more than alpha cells at this age and later ages. Serum Insulin level increased with age progress while serum glucagon level decreased.

The results of immunohistochemistry through the use of a special kit to detect proliferating cells showed a strong activity of proliferating cells of the endocrine secretory units as well as the ductal system at the age of seven days and fourteen days, while it was noted that the percentage of positive reaction decreased to a large degree in later ages.

The Viva committee was chaired by Prof. Dr. Asmaa Abdul-Aziz Ali, University of Mosul / College of Education for Pure Science and the membership of Prof. Dr. Saevan Saad Fadhil / University of Mosul / College of Veterinary Medicine, Asst. Prof. Dr. Siraj Muneer Alkafagy / Al-Qasim Green University/ College of Veterinary Medicine, Asst. Prof. Dr. Fatima Qasim Mohammad, University of Mosul / College of  Science, Asst. Prof. Dr. Baidaa Abdul-Aziz Mohammad/University of Mosul /College of Education for Pure Science and under the supervision and membership of Asst. Prof. Dr. Ammar  Ghanim Alhaaik/ University of Mosul/College of Veterinary Medicine .