College of Education for Pure Science

PH.D. Dissertation Viva in the College of Education for Pure Science entitled ” Spectrophotometric and Fluorometric Determination of Some Drug Compounds Using The basic dyes pyronin G, Janus green B and acidic dyes Phloxine B, Erythosine B and Alkali blue 4B”

The College of Education for Pure Science, University of Mosul, has done the PH.D. Dissertation Viva entitled ” Spectrophotometric and Fluorometric Determination of Some Drug Compounds Using The basic dyes pyronin G, Janus green B and acidic dyes Phloxine B, Erythosine B and Alkali blue 4B”,

On Tuesday, August 1, 2023, the College staff including the respected Dean of the College, Assistant Professor Dr. Qais Ismail Ibrahim, the Honorable Scientific Associate and Administrative Associate, the Honorable Head of the Department of Chemistry, and a number of the college’s teachers were attended the viva.

The aim of this study, presented by the PH.D. Student Rawaa Abdulaleem Ahmed in the Department of Chemistry, was to develop new methods characterized by ease, accuracy, selectivity, high sensitivity, and low cost, and to estimate the drug compounds used in the study, since these drugs were widely prescribed and effective in treating different for the pathological conditions. By following up the published researches we noticed a tendency for the researchers use of organic dyes in estimating chemical compounds in general and medicinal compounds in particular.Two types of dyes were used:

1. Basic dyes (cationic dyes) in which the chromophore (color carrier) is part of the positive ion (usually an amino salt or an ionized amine group) and which can be reacted (pyronine G and Janus green B) with a drug of high negative intensity (enoxaparin sodium) and the formation of ultra-molecular ionic complexes to be estimated spectrophotometrically or fluorometrically.

2. Acid dyes (anionic dyes) in which the chromophore is a part of the negative ion, which is the sodium salt of a sulphonic acid or a carboxylic acid. The principle of estimation depends either on the ability of the dyes (phloxine B and Erythrosine B) to the formation of ionic complexes with the basic drugs, and to path the complexes formation spectrophotometrically and fluorometrically,or on the possibility of bleaching the color of the dye (alkaline blue 4B) and oxidizing the drug compounds with the appropriate oxidizing agent and indirectly assessing it spectrophotometrically. Finally, the greenness evaluation of the proposed spectrophotometric and spectrofluorimetric methods for the determination of the studied drug compounds was discussed by application of two assessment tools including: AGREE tool which provides greenness profiles as a numerical values(scores) between 0.71 to 0.79 and GAPI in which pentagrams showed the proposed methods possessed the highest number of green zones and lowest number of red zones. In conclusion, the obtained results from the AGREE and GAPI tools indicated that the highest greenness and environmental friendliness of the proposed methods.

The Viva committee was chaired by Prof. Dr. Nabeel Sabeeh Othman / University of Mosul / College of Science and the membership of Prof. Dr. Moath Abdullah Najim / College of Agriculture and Forestry, University of Mosul , Asst. Prof. Dr. Subhi Mohsin Jarullah / University of Mosul / College of Education for Pure Science, Asst. Prof. Dr. Khalida Mohammed Omar / University of Mosul / College of Science, Asst. Prof. Dr. Asmaa Ahmed Mohammed / College of Education for Pure Science, University of Tikrit and under the supervision and membership of Prof. Dr. Elham Sadullah Salih / University of Mosul / College of Education for Pure Science.