College of Education for Pure Science

Master thesis Viva in the College of Education for Pure Science entitled ” Study the effect of some willow bark extracts on lipase enzyme ”

The College of Education for Pure Science, University of Mosul, has done the Master thesis Viva entitled ” Study the effect of some willow bark extracts on lipase enzyme “,

On Wednesday, August 30, 2023, the College staff including the respected Dean of the College, Assistant Professor Dr. Qais Ismail Ibrahim, the Honorable Scientific Associate and Administrative Associate, the Honorable Head of the Department of Chemistry, and a number of the college’s teachers were attended the viva.

This study, presented by the master student Fatima Murhaf Mohammed Alrushdi in the Department of Chemistry, includes preparation of some willow tree bark extracts and the ability of inhibiting the lipase of fungus Aspergillus niger. The aqueous extract showed the highest percentage of inhibition 54.35%,

The study also involved partial purification of lipase from the ovine pancreas using some biochemical techniques. It was discovered that crude enzyme had a specific activity of 0.008 enzyme units/mg protein.

Ethyl acetate extract displayed uncompetitive inhibition, with decreasing of Vmax and Km values from 4.17 to 1.14 enzyme units and 12.5 to 4.35 mM respectively. Aspirin demonstrated competitive inhibition, and it was noted that Vmax value remained stable at 2 enzyme units, while Km value increased from 1.05 to 7.7 Mm.

The study also involved preparation of the compound isopropyl salicylate, which was prepared in a laboratory, and identified by infrared spectroscopy and nuclear magnetic resonance. The prepared compound was tested for lipase inhibition, and the highest percentage of inhibition was found to be 85%. The compound demonstrated competitive inhibition when inhibition type was examined using the concentration that gave approximately half inhibition, as the value of Vmax remained constant at 1.78 enzyme units and Km increased from 2.04 to 5.26 mM.

The Viva committee was chaired by Prof. Dr. Othman Rashid Hamid /College of Education / Samarra University and the membership of Asst. Prof. Dr. Muhammad Abdul Hadi Jassim / University of Mosul / College of Education for Pure Science, Asst. Prof. Dr. Lilas Farhan Budaiwi/ University of Mosul / College of Education for Girls, and under the supervision and membership of Prof. Dr. Omar Younis Muhammad / University of Mosul / College of Education for Pure Science.