College of Education for Pure Science

Master thesis Viva in the College of Education for Pure Science entitled ” Isolation and extraction of some active compounds and study of their biochemical effects on laboratory rats ”

The College of Education for Pure Science, University of Mosul, has done the Master thesis Viva entitled ” Spectral Thermodynamic Study for The Interactions of Some Drug Compounds With two Reagents Diazotize Isolation and extraction of some active compounds and study of their biochemical effects on laboratory rats”,

On Tuesday, September 12, 2023, the College staff including the respected Dean of the College, Assistant Professor Dr. Qais Ismail Ibrahim, the Honorable Scientific Associate and Administrative Associate, the Honorable Head of the Department of Chemistry, and a number of the college’s teachers were attended the viva.

This study, presented by the master student Muthanna Salahuddin Ibrahim Ali in the Department of Chemistry, aimed to know the protective effect of each of the aqueous extract of hylocereus undatus and the active compounds isolated from it (vitamin C and gallic acid) to reduce the effect of arthritis induced by Freud’s Complete Adjuvant in white male rats by studying some changes in biochemical and histological parameters as the study included several parts:

The first part of the study measured studied some of biochemical variables in blood serum of male white rats and determined the median lethality dose of Freud’s Complete Adjuvant in tumor development of rheumatoid arthritis, which was (0.1ml).

The second part included the water extraction process of dragon fruit peels (hylocereous undatus) and the isolation of the active compounds (vitamin C and gallic acid) by using high-performance liquid chromatography technology (HPLC) and use the infrared spectroscopy technology(IR) for the isolated compounds to verify them by comparing them with ( Standard) for isolated compounds.

The third part divided the animals into six groups, which are (negative control group, positive control group with 250 mg/kg aqueous extract group, 500 mg/kg aqueous extract group, 200 mg/kg vitamin C group, and 40 mg/kg gallic acid group.

Several standard ready analyzes were used in the study to measure (RF, Hcy, interleukin-6, vitamin B12 , Gpx , ANA ,Anti-ccp ) as well as (malonaldehyde and glutathione) in tissue and blood serum were estimated using manual methods of preparation .

The fourth part included a comparison between the experimental groups :

It is concluded from this study that the use of the aqueous extract and the active compounds isolated from the dragon fruit plant showed a protective effect that reduced the damage resulting from the induction of arthritis by (CFA), as well as an improvement in the tissues of the kidney, liver and joint.

The Viva committee was chaired by Prof. Dr. Nashwan Ibrahem Abbou / University of Mosul / College of Education for Pure Science and the membership of Asst. Prof. Dr. Intsar Ghanim taha / University of Mosul / College of Basic Education, Asst. Prof. Dr. Nagham Qasim Kazem / University of Tikrit / College of Sciences, and under the supervision and membership of Assist. Prof. Dr. Luma Abd Almunim Baker / University of Mosul / College of Education for Pure Science.