The master thesis entitled “isolation and evaluation of the level of muramidase and some biochemical variables in patients with Crohn’s disease” was discussed at the Department of Chemistry, College of Science, University of Mosul, on Tuesday, October 7, 2025.

The dean of the College, Dr. Hiyam Adel Ibrahim, attended part of the discussion, along with the head of the Department of chemistry, Dr. Salem Jassim Mohammed, and a number of college teachers.

The thesis was presented by the student Esraa Tahsin Ali from the Department of chemistry, the study dealt with measuring the level of muramidase enzyme in Crohn’s disease patients and comparing it with healthy people, as well as measuring the levels of some cytokines and biochemical elements, in addition to isolating and purifying the muramidase enzyme, determining its molecular weight, and conducting a genetic study to investigate any genetic changes or mutations that may be associated with the disease using RT-PCR technology.

The study aimed to:

Assessment of the level of muramidase enzyme (Muramidase) in the blood serum of patients, its isolation and purification using advanced biotechnologies, and determine its relationship with Crohn’s disease.

Measuring the levels of such inflammatory factors as tumor necrosis factor – alpha (TNF-α), interferon – Kama (IFN-γ), Interleukin-12 and Interleukin-23, along with a number of chemobiotic variables, and studying their correlation with the pathological condition.

Analysis of the influence of clinical factors such as smoking, duration of injury, age, family history, obesity on the level of the enzyme and the studied variables.

Conduct a comparative genetic study between patients and healthy people to detect any mutations in the genes responsible for the production of the enzyme.

The discussion committee consisted of:

Dr. Zeina Abdel Moneim Mohamed Saleh-president

Dr. Saba Zaki Mahmoud Khader-member

Mr. Mahmoud Ahmed Mohamed Fakhri-member

Under the supervision and membership of assistant professor Dr. Amal Taha Yassin Ramla.

Relevance of the study to the Sustainable Development Goals

This study is in line with the United Nations Sustainable Development Goals, in particular:

The third goal: good health and well-being, by supporting biomedical research that contributes to improving the diagnosis and treatment of chronic diseases.

The fourth goal: quality education, by promoting scientific research and encouraging students to innovate in health fields.

The ninth goal: industry, innovation and infrastructure, through the use of modern biotechnologies in enzyme analysis and genetic studies.

We wish our dear students continued success and success .

Government information and Communication Division

Tuesday, October 7, 2025